RESUMO: Descreve-se um caso de rinite por Exserohilum rostratum em um caprino, sem raça definida, fêmea, três anos, com diminuição do apetite, dispneia e deformidade facial, com evolução de aproximadamente dois meses. Durante a necropsia, observou-se que na cavidade nasal havia área focal extensa, amarelo acastanhada, irregular, ulcerada e friável, que drenava secreção purulenta acometendo o vestíbulo nasal e estendendo-se da porção caudal da concha dorsal, associada a obstrução do meato nasal, destruição do septo nasal e osso nasal palatino e vômer. Histologicamente, a lesão foi caracterizada como rinite e osteomielite piogranulomatosa e necrosante, multifocal a coalescente, aguda, crônica, associada a uma miríade de estruturas fúngicas. Morfologicamente, as hifas foram caracterizadas por paredes finas, discretamente tortuosas e septadas medindo 2 a 6 μm de diâmetro. Observamos algumas cadeias de conídios ou grupos com 8 a 10 μm de diâmetro e clamidoconídios pigmentados medindo de 7,5 a 15 μm de diâmetro. Foram positivos na histoquímica especial de Fontana Masson, Nitrato de Metenamina de Prata de Grocott e Ácido Periódico de Schiff. O agente foi determinado por meio de isolamento microbiológico associado à identificação molecular. A rinite piogranulomatosa causada por E. rostratum em caprinos é inédita, sendo o primeiro isolamento do agente em animais no Brasil e o primeiro caso de feo-hifomicose nasal na espécie caprina.

ABSTRACT: We described a case of rhinitis case caused by Exserohilum rostratum in a 3-year-old, mixed breed, afemale goat, presenting with decreased appetite, dyspnea, and face deformity, with evolution of approximately 2 months. Necropsy revealed an extensive brownish yellow, irregular, ulcerated, and friable focal lesion in the nasal cavity. Purulent secretion drained from the lesion, affecting the nasal vestibule and extending the caudal portion of the dorsal concha, associated with nasal meatus obstruction, destruction of the nasal septum, and nasal bone palatine and vomer. Histologically, the lesion was characterized as rhinitis and pyogranulomatous and necrotizing osteomyelitis (multifocal to coalescent, sharp, and chronic) and associated with a myriad of fungal structures. Morphologically, the hyphae were characterized by thin, slightly tortuous, and rarely septate walls (2-6 μm diameter), multiple chains of individual conidia or in groups (8-10 μm diameter), and pigmented chlamydoconidia (7.5-15 μm diameter). They showed positive results for the special histochemical techniques such as Fontana Masson, methenamine silver nitrate by Grocott, and periodic acid by Schiff. E. rostratum was identified as the causative agent using microbiological isolation associated with molecular identification. Pyrogranulomatous rhinitis caused by E. rostratum in goats is unprecedented, being the first isolation of the agent in animals in Brazil and the first case of nasal phaeohyphomycosis in the goats.

ABSTRACT. The addition of organic and inorganic amendments can improve soil structure and reduce soil compaction. In this context, this study aimed to evaluate whether the application of amendments reduces penetration resistance (PR) in the short term and describe the spatial variability of PR in the surface horizon of an Aquic Argiudoll under no-tillage in northeast Argentina. Four treatments, consisting of surface applications of 7.5 Mg ha−1 poultry litter (PL), 3.0 Mg ha−1 gypsum (G), the combination of PL+G, and untreated control (T), were arranged in a complete randomized block design with three replications. Two more treatments were added to the experiment 12 months later, consisting of PL reapplications on half of the surface of the PL+G and PL treatments (PL+G+PL and PL+PL, respectively) in a split-plot design with three replications in 4×20-m plots. PR was determined in the field with an Eijkelkamp penetrologger following a 2-m long transect perpendicular to the sowing direction at 10 different spots separated 0.2 m from each other. The spatial variability was quantified for each treatment using semivariograms. The highest PR was observed in the T treatment (1.96 MPa) and the lowest PR in PL+G+PL (0.21 MPa). All treatments showed a high spatial dependence (94.9 to 99.9%). Treatments with PL reapplication (PL+PL and PL+G+PL) showed profiles with lower PR and more homogeneous kriging maps. PL reapplication on PL treatments showed no effects on PR values. However, PL reapplication on the PL+G treatment led to positive effects in all PR ranges. Thus, the PL+G+PL treatment, which had the highest PR values, showed a decrease in PR from 54.17 to 6.65% with the reapplication 12 months later. The addition of organic and inorganic amendments reduced specific compacted soil areas on the surface horizon of an Aquic Argiudoll under no-tillage.

ABSTRACT Objective: Test if the MRI FAST1.2 protocol can detect extra-pituitary midline structural brain abnormalities in patients with ectopic posterior pituitary (EPP), and highlighting their radiological-laboratory correlations. Subjects and methods: Cross-sectional study of patients with EPP and control group. All individuals were submitted to FAST1.2, which combines the FAST1 protocol developed by our group with 3D T2DRIVE imaging. Results: We evaluated 36 individuals with EPP and 78 as control group. Pituitary stalk (PS) was identified in 7/36 patients in EPP group by FAST1, and in 24/36 patients in FAST1.2 (p < 0.001). FAST1 failed to detect PS in one individual in the control group, while the FAST1.2 defined the PS in all individuals. In EPP group, eleven had interhypothalamic adhesion (IHA), three septo-optic dysplasia, and one cerebellar malformation. We didn’t observe higher frequency of panhypopituitarism or developmental delay in patients with IHA. In control group, three had pars intermedia cysts, one hydrocephalus, and one hypothalamic hamartoma. Conclusions: FAST1.2 allows confident recognition of midline structural abnormalities, including the pituitary stalk and IHA, thereby making MRI acquisition faster and with no need for contrast administration. IHA could be associated with defects in neuronal migration, as occur in patients with EPP, with no clinical significance.

Resumo O objetivo deste trabalho foi avaliar a eficácia e a seletividade de herbicidas aplicados em pré-emergência no cultivo de melão (Cucumis melo). Os experimentos foram conduzidos de 2017 a 2018, no delineamento de blocos ao acaso, com quatro repetições, no estado do Rio Grande do Norte, Brasil. Os tratamentos consistiram na aplicação pré-emergencial de: 35 g ha-1 i.a. de flumioxazin, 25 g ha-1 i.a. de flumioxazin, 250 g ha-1 i.a. de sulfentrazone, 480 g ha-1 i.a. de metribuzin, 240 g ha-1 i.a. de oxyfluorfen, 240 g ha-1 i.a. de oxyf luorfen + 960 g ha-1 i.a. de S-metolachlor, 360 g ha-1 i.a. de clomazone e 360 g ha-1 i.a. de clomazone + 240 g ha-1 i.a. de oxyfluorfen; em 2018, o metribuzin não apresentou seletividade e foi substituído por 480 g ha-1 i.a. de ametrina and 1.600 g ha-1 i.a. de diuron. Foram avaliados a produtividade e a qualidade dos frutos, bem como o nível de controle e a massa seca das plantas daninhas. Os herbicidas metribuzin, ametrina e diuron causaram a morte das plantas de melão. A melhor eficiência no controle das plantas daninhas foi obtida com a mistura e clomazone + oxyfluorfen, seguida de oxyfluorfen, oxyfluorfen + S-metolachlor e clomazone. Os tratamentos com oxyfluorfen, oxyfluorfen + S-metolachlor e clomazone + oxyfluorfen não foram considerados seletivos. O clomazone foi o único herbicida seletivo nos dois anos de avaliação e pode ser alternativa para o controle de plantas daninhas na cultura do melão.

Abstract The objective of this work was to evaluate the effectiveness and selectivity of herbicides applied at preemergence to the melon (Cucumis melo) crop. The experiments were carried out from 2017 to 2018, in a randomized complete block design, with four replicates, in the state of Rio Grande do Norte, Brazil. The treatments consisted of the preemergence application of: 35 g ha-1 a.i. flumioxazin, 25 g ha-1 a.i. flumioxazin, 250 g ha-1 a.i. sulfentrazone, 480 g ha-1 a.i. metribuzin, 240 g ha-1 a.i. oxyfluorfen, 240 g ha-1 a.i. oxyfluorfen + 960 g ha-1 a.i. S-metolachlor, 360 g ha-1 a.i. clomazone, and 360 g ha-1 a.i. clomazone + 240 g ha-1 a.i. oxyfluorfen; in 2018, metribuzin did not show selectivity and was substituted by 480 g ha-1 a.i. ametryn and 1,600 g ha-1 a.i. diuron. Fruit yield and quality, as well as weed control and dry mass, were evaluated. The metribuzin, ametryn, and diuron herbicides caused melon plant death. The best efficiency in weed control was obtained with the mixture clomazone + oxyfluorfen, followed by oxyfluorfen, oxyfluorfen + S-metolachlor, and clomazone. The treatments with oxyfluorfen, oxyfluorfen + S-metolachlor, and clomazone + oxyfluorfen were not considered selective. Clomazone was the only selective herbicide in the two years of evaluation and can be an alternative for weed control in melon crops.

ABSTRACT. The management of weeds in melon is hindered by the slow initial growth of the crop, favouring weed infestation and reducing production. During the years 2016 and 2017, weed interference periods were evaluated in two melon hybrids, yellow and frog skin, to determine the time of weed control in the crop. Four-parameter logistic regression was used to determine the critical weed control period (CPWC). The weed species with the highest occurrences in 2016 were Merremia aegyptia, Senna obtusifolia, and Urochloa plantaginea. In 2017, there was a predominance of M. aegyptia, indicating the possibility of successive cultivation, favouring the predominance of species with growth habits similar to melons. The increase in the period of living with weeds reduced the productivity and quality of melon fruits. Coexistence with weeds throughout the cycle reduced productivity by about 50%. The CPWC was 15 to 58 and 10 to 45 for frog skin and 12 to 52 and 4 to 50 days for yellow melon in 2016 and 2017, respectively, considering a productivity loss of 5%. These results underscore the importance of adopting strategies that allow melons to grow free from the presence of weeds, especially before they are fully established in the growing area.

Abstract Objective was evaluated the therapeutic effect of Juglans regia (J) and Zingiber officinale (Z) extracts, alone or associated (Z75% + J25%, Z50% + J50% and Z25% + J75%) applied on planktonic cultures and biofilms of Propionibacterium acnes, Staphylococcus epidermidis and Staphylococcus aureus, as well as analyzing the cytotoxic effects of plant extracts on mouse macrophages (Raw 264-7). Broth microdilution assay was performed (M7-A6 - CLSI). Anti-biofilm activities and cytotoxicity on Raw 264-7 were studied using MTT assay and scanning electron microscopy. ANOVA with post-hoc Tukey HSD applied for parametric data and Kruskal-Wallis with Conover-Iman test, for non-parametric (p<0.05). On P. acnes biofilm, Z50% + J50% reduced 46.9% in 5 min and Z25% + J75% reduced 74.1% in 24hs. On S. aureus, Z75% + J25% reduced 23.1% in 5 min Z25% +J75% reduced 79.4% in 24hs. On S. epidermidis, Z75% + J25% reduced 74.6% in 5 min and 82.05% in 24 h. The treatments on macrophages for 24 h promoted a maximum reduction by 14,5% for groups of extracts associations. On multispecies biofilm, Z75%+J25% reduced 84.3% in 24 h. In conclusion association of glycolic extracts provided therapeutic effect, demonstrated antimicrobial activity and low cytotoxicity.

Resumo: Este artigo visa apresentar contribuições teóricas e práticas à educação, considerando o ensino de conteúdos escolares como uma dificuldade concernente à comunicação. Para isso, são realizadas duas operações: a análise teórica das ideias de John Dewey sobre o significado educacional da comunicação e da imaginação e a apresentação de um experimento de pensamento que simula dramaticamente uma situação prática - um curso de formação de professores cujo objetivo é ensinar tais ideias deweyanas utilizando as noções de drama e experimento de pensamento. As conclusões do artigo discutem a contribuição do experimento de pensamento para instituir valores fundamentais da prática docente.

Abstract: This article aims to present theoretical and practical contributions to education, considering the teaching of school subjects as a difficulty concerning communication. For this, two operations are carried out: the theoretical analysis of John Dewey’s ideas about the educational meaning of communication and imagination, and the presentation of a thought experiment that dramatically simulates a practical situation - a teacher training course whose objective is to teach such Deweyan ideas using the notions of drama and thought experiment. The conclusions of the article discuss the contribution of the thought experiment to establish fundamental values of teaching practice.

Resumen: Este artículo tiene como objetivo presentar aportes teóricos y prácticos a la educación, considerando la enseñanza de los contenidos escolares como una dificultad de comunicación. Para ello, se realizan dos operaciones: el análisis teórico de las ideas de John Dewey sobre el significado educativo de la comunicación y de la imaginación y la presentación de un experimento de pensamiento que simula dramáticamente una situación práctica -un curso de formación docente cuyo objetivo es enseñar tales ideas de Dewey, utilizando las nociones de drama y de experimento de pensamiento. Las conclusiones del artículo discuten la contribución del experimento mental para establecer valores fundamentales de la práctica docente.

Resumo A qualidade do solo refere-se à avaliação das propriedades químicas, físicas e biológicas do solo e tem sido um assunto explorado dentro das linhas de pesquisas agronômicas em decorrência de ações antrópicas que, muitas vezes, causam degradação. Objetivou-se pesquisar, entre os atributos físicos e biológicos estudados, aqueles que melhor expliquem, linear e espacialmente, a variabilidade do solo cultivado com soja na estação chuvosa e em pousio na estação seca no sistema agrossilvipastoril. O trabalho foi conduzido no ano agrícola 2015/2016, em Ipameri, Goiás. Analisaram-se as distribuições espaciais e as correlações entre alguns atributos indicadores da qualidade do solo: carbono da biomassa microbiana (CBM), respiração microbiana do solo (C-CO2), quociente metabólico (qCO2) e umidade do solo (UG), na profundidade de 0,00-0,10 m, nos períodos de chuva e seca. Instalou-se no local, uma malha geoestatística, onde foram coletados 52 pontos. Foi realizada a análise descritiva dos dados, a fim de obter correlações lineares, simples e múltiplas, entre os atributos. Os semivariogramas foram modelados a fim de obter as respectivas krigagens e validações cruzadas. Estabeleceram-se as co-krigagens de interesse. Os atributos microbiológicos do solo foram bons indicadores de zonas de manejo específico, apresentando maior dependência espacial no período chuvoso. O qCO2 apresentou relação direta com C-CO2 no período chuvoso, enquanto CBM apresentou relação inversamente proporcional tanto no período chuvoso, como no seco. O qCO2 foi o atributo que melhor representou, linear e espacialmente, a variabilidade do solo sob o sistema ILPF, refletindo as zonas de maior eficiência do uso do substrato pelos organismos do solo.

Abstract Soil quality refers to the assessment of the chemical, physical and biological properties of the soil and has been a subject explored within the lines of agronomic research because of anthropic actions that often cause degradation. The objective was to research, among the studied physical and biological attributes, those that best explain, linearly and spatially, the variability of the soil cultivated with soybeans in the rainy season and fallow in the dry season in the agrosilvopastoral system. The work was carried out in the 2015/2016 agricultural year, in Ipameri, Goiás. The spatial distributions and correlations between some soil quality indicator attributes were analyzed: microbial biomass carbon (CBM), soil microbial respiration (C-CO2), metabolic quotient (qCO2) and soil moisture (UG), at a depth of 0.00-0.10 m, during periods of rain and drought. A geostatistical grid was installed, where 52 points were collected. Descriptive analysis of the data was carried out to obtain linear, simple and multiple correlations between the attributes. The semivariograms were modeled to obtain the respective krigings and cross-validations. The co-krigages of interest were established. The microbiological attributes of the soil were good indicators of specific management zones, showing greater spatial dependence in the rainy season. qCO2 was directly related to C-CO2 in the rainy season, while CBM was inversely proportional in bothrainy and dry periods. The qCO2 was the attribute that best represented, linearly and spatially, the variability of the soil under the ILPF system, reflecting the zones of greatest efficiency in the use of the substrate by soil organisms.

RESUMO: A hérnia reticular diafragmática é uma alteração congênita ou adquirida resultante da protrusão do retículo para o interior da cavidade torácica. Em ruminantes, lesões no músculo diafragmático devido a penetração de objetos metálico pontiagudos constitui a causa mais comum dessa enfermidade. Portanto, diante dos poucos relatos a cerca dessa enfermidade nos animais da espécie bovina, este estudo tem como objetivo descrever os achados clínicos, laboratoriais, anatomopatológicos e dar ênfase especial no diagnóstico ultrassonográfico de cinco bovinos acometidos com hérnia reticular diafragmática. Os dados laboratoriais foram analisados utilizando-se media e desvio padrão e os achados dos exames clínico, ultrassonográfico e anatomopatológicos foram avaliados através de estatística descritiva. Clinicamente os animais exibiam desidratação em variados graus, distensão abdominal, timpania e alterações na motilidade ruminal. Além de alterações cardiorrespiratórias como sopro, dispneia e abafamento dos sons pulmonares. O exame laboratorial revelou leucocitose por neutrofilia e hiperfibrinogenemia. As imagens ultrassonográficas revelaram retículo no interior da cavidade torácica adjacente ao pulmão e coração, porém nenhuma motilidade reticular foi observada. As lesões anatomopatológicas confirmaram os achados dos exames ultrassonográficos. Dessa maneira, este trabalho demonstrou que a ultrassonografia foi eficiente no diagnóstico da hérnia reticular diafragmática nos animais da espécie bovina.

ABSTRACT: A reticular diaphragmatic hernia is a congenital or acquired alteration resulting from protrusion of the reticulum into the thoracic cavity. In ruminants, lesions to the diaphragmatic muscle, due to penetration of sharp metallic objects, is the most common cause of this disease. Therefore, given the low number of reports on this disease in the bovine species, the current study aims to describe the clinical, laboratory, and anatomopathological findings, with special emphasis on the ultrasound diagnosis of five cattle with reticular diaphragmatic hernia. The laboratory data were analyzed using mean and standard deviation, and clinical, ultrasound, and pathological findings were evaluated using descriptive statistics. Clinically the animals exhibited varying degrees of dehydration, abdominal distension, tympany, and alterations in ruminal motility, in addition to cardiorespiratory alterations such as murmur, dyspnea, and muffling of lung sounds. The laboratory examination showed neutrophilic leukocytosis and hyperfibrinogenemia. The ultrasonographic images demonstrated reticulum inside the thoracic cavity adjacent to the lung and heart, although no reticular motility was observed. The pathological lesions confirmed the findings of the ultrasound exams. Thus, the current study demonstrated that ultrasonography was efficient in diagnosing reticular diaphragmatic hernia in the bovine species.

Resumo: Este artigo analisa a metáfora como estratégia discursiva destinada a explicar e a difundir os conceitos que sustentam as doutrinas em geral e as doutrinas educacionais em particular. Examina a metáfora cultivo como metáfora fundamental da educação, bem como a sua suposta origem na Sofística, e propõe substituí-la pela metáfora navegação. Esta substituição permite atribuir novos significados tanto à prática dos sofistas quanto ao ofício de educar no decorrer da história e na atualidade, impondo desafios à formação inicial e continuada de professores.

Abstract: This paper analyzes the metaphor as a discursive strategy to explain and disseminate the concepts that support doctrines in general and educational doctrines in particular. It examines the Cultivation metaphor as a fundamental metaphor for education, as well as its supposed origin in Sophistry, and proposes to replace it with the Navigation metaphor. This substitution makes it possible to assign new meanings both to the practice of the sophists and to the profession of educating today and throughout history, imposing challenges on the training and continuing education of teachers.

RESUMO: A ingestão de corpos estranho de origem metálica e/ou pontiagudos desencadeia em bovinos, quadros de Reticuloperitonite Traumática e suas sequelas. Dentre as quais podemos destacar a retículo esplenite traumática cuja letalidade é elevada, embora a mesma apresente uma baixa frequência na clínica de ruminantes. Portanto, baseado na escassez de informações sobre esta enfermidade, este trabalho teve por objetivo avaliar os achados clínicos, laboratoriais, ultrassonográficos e anatomopatológicos de 30 bovinos adultos diagnosticados com retículo esplenite traumática. Os achados clínicos, ultrassonográfico e anatomopatológico foram analisados por meio de estatística descritiva, e os dados laboratoriais foram avaliados utilizando-se as medidas de tendência central. Clinicamente os animais apresentaram desidratação e alterações no comportamento, apetite e na motilidade ruminal. Os achados hematológicos revelaram leucocitose (37077.17±25004.59cell/μL) por neutrofilia com desvio à esquerda regenerativo e hiperfibrinogenemia (1130±364.98mg/dL). O exame ultrassonográfico possibilitou a visualização de filamentos móveis e ecogênicos que corresponderam à presença de aderências fibrinosas, observou-se também, deslocamento do retículo e irregularidade no seu contorno além das alterações na quantidade, padrão e amplitude das contrações reticulares. Permitiu ainda, a constatação de alterações esplênicas como abscessos que foram caracterizados como estruturas circulares de variados tamanhos delimitada por capsula contendo no seu interior conteúdo de ecogenicidade variável. Trombose da veia esplênica e dobramento do baço. Os resultados obtidos nesse trabalho, indicaram que a retículo esplenite traumática causa sinais clínicos inespecíficos, severas alterações laboratoriais e principalmente que a ultrassonografia é um método eficiente para o diagnóstico dessa enfermidade uma vez que as lesões anatomopatológicas confirmaram os achados ultrassonográficos.

ABSTRACT: Ingestion of metallic and/or sharp foreign bodies triggers cases of traumatic reticuloperitonitis and its sequelae in cattle. Among these sequelae, we can highlight traumatic reticulosplenitis, that has high mortality, although its frequency in the ruminant medicine is low. Therefore, based on the scarcity of information on this disease, the current study aimed to evaluate the clinical, laboratory, ultrasonographic, and pathological findings of 30 adult cattle diagnosed with traumatic reticulosplenitis. Clinical, ultrasound, and anatomopathological findings were analyzed using descriptive statistics and laboratory data were evaluated using measures of central tendency. Clinically the animals presented dehydration and alterations in behavior, appetite, and ruminal motility. Hematological findings revealed neutrophilic leukocytosis (37077.17±25004.59cell/μL) with regenerative left shift and hyperfibrinogenemia (1130±364.98mg/dL). The ultrasound examination enabled visualization of mobile and echogenic filaments that corresponded to the presence of fibrin adhesions. Displacement of the reticulum and irregularity in its contour, as well as alterations in the quantity, pattern, and amplitude of reticular contractions were also observed. Splenic alterations such as abscesses were found, characterized as circular structures of varying sizes delimited by capsules containing variable echogenicity. Splenic vein thrombosis and spleen folding were also observed. The results obtained in the current study indicated that traumatic reticulosplenitis causes nonspecific clinical signs, severe laboratory alterations and, mainly, that ultrasound is an efficient method for the diagnosis of this disease, since the anatomopathological lesions confirmed the ultrasound findings.

RESUMO Populações do patógeno da mela da soja (Rhizoctonia solani AG-1 IA) são altamente diferenciadas geneticamente ao longo de um gradiente latitudinal nas mais importantes regiões de cultivo de soja do Brasil. Entretanto, os processos evolutivos que guiaram a adaptação regional são ainda desconhecidos. O objetivo deste trabalho foi avaliar a importância relativa da variação genética neutra e da seleção natural sobre a divergência e adaptação regional de populações de R solani AG-1 IA da soja. Para tanto, comparou-se a diferenciação fenotípica por caracteres quantitativos (QST) e a diferenciação genética neutra (baseada em dados de microssatélites) entre três pares de populações (FST). Como medidas de respostas fenotípicas do fungo (caracteres quantitativos), estimou-se a tolerância ao estresse de temperatura e a tolerância a um fungicida cúprico de amplo espectro de ação (oxicloreto de cobre) sob condições ótimas (25 °C) e de temperatura elevada (33,5 °C). No geral houve aumento da variância genética com um efeito positivo sob a herdabilidade para tolerância ao fungicida cúprico na temperatura de estresse. As diferenças genéticas entre populações foram os principais determinantes da adaptação térmica em R. solani AG-1 IA (h2 > 0.70). A análise da estrutura genética neutra (FST) indicou subdivisão entre os três pares de populações. Embora a comparação de FST com os dados QST entre pares de populações não tenha seguido um único padrão, a maioria dos valores de QST estimados não diferiram significativamente de FST, indicando que, para os caracteres quantitativos estudados, a neutralidade (ou evolução neutra) teve importante papel na adaptação regional das populações do patógeno.

ABSTRACT Populations of the soybean leaf blight pathogen (Rhizoctonia solani AG-1 IA) are highly genetically differentiated along a latitudinal gradient in the major soybean growing regions of Brazil. However, the evolutionary processes leading to regional adaptation are still unknown. The objective of this study was to evaluate the relative importance of neutral genetic variation and natural selection on the divergence and regional adaptation of populations of the soybean-infecting pathogen R. solani AG-1 IA. Therefore, we compared the phenotypic differentiation in quantitative traits (QST) and the neutral genetic differentiation (FST, based on microsatellites data) among three pairs of populations. As measures of phenotypic responses of the fungus (quantitative traits), we estimated the tolerance to temperature stress and the tolerance to a broad-spectrum fungicide (copper oxychloride) under optimal (25 °C) and high temperature conditions (33.5 °C). In general there was an increase in genetic variance with a positive effect on the heritability for tolerance to copper fungicide under temperature stress. The genetic differences among populations were the main determinants of thermal adaptation in R. solani AG-1 IA (h2 > 0.70). The analysis of neutral genetic structure (FST) indicated subdivision between the three pairs of populations. Although population pairwise comparisons between FST and QST values did not follow a single pattern, the majority of QST values did not differ significantly from FST, indicating that, for the quantitative characters studied, neutrality (or neutral evolution) had a major role in the regional adaptation of R. solani AG-1 IA populations.

RESUMO: O objetivo desse estudo foi determinar, através da reação de imunofluorescência indireta (RIFI, 1:64), a ocorrência de anticorpos IgG para o Toxoplasma gondii e os fatores de risco associados à infecção em rebanhos caprinos do estado de Sergipe, a partir de 675 amostras de sangue de animais de 41 propriedades das três mesorregiões do estado entre os anos de 2013 e 2014. A ocorrência de caprinos soropositivos foi de 30,07% com 90,24% de propriedades com animais soropositivos. A distribuição dos títulos obtidos foi de 37,93%, 11,82%, 17,24%, 18,22% e 17,77% para 64, 128, 512 e 1024 respectivamente. Os fatores de riscos observados foram propriedades que não possuíam instalações (p=0,000, OR=2,30, IC 95%=1,41-3,74), com terrenos alagados (p=0,011, OR=2,94, IC 95%=1,27-6,79), que disponibilizam a ração no chão (p=0,032, OR=1,69, IC 95%=1,04-2,74), em cochos descobertos (p=0,000, OR=1,97, IC 95%=1,32-2,94), alimentação à base de pastagem (p=0,003, OR=3,52, IC 95%=1,53-8,09), com acesso de gatos à fonte de água (p=0,031, OR=1,45, IC 95%=1,03-2,04) e que introduziram novos reprodutores nos últimos cinco anos (p=0,036, OR=1,58, IC 95%=1,02-2,74).

ABSTRACT: The objective was to determine, through indirect immunofluorescence reaction (RIFI, 1:64), the occurrence of IgG antibodies to Toxoplasma gondii and the risk factors associated with infection in goats in the state of Sergipe. To this study were used 675 samples of blood of animals from 41 farms of the three state mesoregions from 2013 to 2014. The occurrence of seropositive goats was 30.07%, with 90.24% of farms with seropositive animals. The distribution of titers obtained was 37.93%, 11.82%, 17.24%, 18.22%, and 17.77% for 64, 128, 512 and 1024 respectively. The risk factors observed were farms that did not have facilities (p=0.000, OR=2.30, CI 95%=1.41-3.74), with flooded soils (p=0.011, OR=2.94, CI 95%=1.27-6.79), which provided feed on the ground (p=0.032, OR=1.69, CI 95%=1.04-2.74), in uncovered cages (p=0.032, OR=1.69, CI 95%=1.04-2.74), pasture-based feed (p=0.003, OR=3.52, CI 95%=1.53-8.09), with access from cats to (p=0.031, OR=1.45, CI 95%=1.03-2.04) and introduced new breeders in the last five years (p=0.036, OR=1.58, CI 95%=1.02-2.74).

ABSTRACT The Darcy-Weisbach equation is the most recommended equation for determining the pressure loss in pressurized pipes because of its wide applicability. However, one of the largest obstacles to implementing this equation is the friction factor (f) calculation. This factor can be precisely determined using the Colebrook equation, which is implicit. Thus, the objective of this study was to compare six explicit equations for calculating the Darcy-Weisbach friction factor with the implicit Colebrook equation based on the relative error. Based on the results, the equations of Vatankhah and Offor & Alabi were the most highly recommended. These six explicit formulas showed a mean relative error of less than ± 1% compared to the Colebrook equation, except for the Swamee and Jain equation, for which the laminar regime generated a mean relative error of 1.83%.